蔡光先教授运用五味异功散合过敏煎治疗小儿过敏性疾病验案举隅

蔡光先系国家级名中医,第五批全国名老中医药专家学术经验继承工作指导老师,博士研究生导师,国家有突出贡献专家,从事中医药教学、科研及临床工作40余载,擅长内科疑难杂症的诊治,临床经验丰富,学术造诣颇深,笔者有幸跟师临证学习,受益匪浅.现择其运用五味异功散合过敏煎治疗小儿过敏性疾病验案3则报道如下,以飨同道.     

蔡连香妇科临床用药规律初探

目的：采用数据挖掘法分析名老中医蔡连香的妇科临床用药规律。方法选择蔡连香临床医案165例、共计337诊次的处方资料,用Excel建立数据库,使用中医诊疗大数据智能分析系统进行数据分析,得出高频药物及高频药物组合,并与蔡连香学术思想及临床经验相印证。结果总结出高频药物10味,高频药物组合7组,与蔡连香认为妇科疾病病机以精血不足、肝脾肾三脏失调为主,临床治疗以益精养血、调肝健脾补肾为主要法则的学术观点一致。结论运用数据挖掘的方法总结蔡连香老师的妇科用药规律,对临床具有一定的指导意义。     

经肛门自然腔道取标本的腹腔镜左结肠癌根治术围术期舒适护理

目的 探讨腹腔镜辅助自然腔道取标本左结肠癌根治术(LA-NOSE)的围手术期舒适护理方法 .方法 总结31例采用蔡氏套管器经肛门自然腔道取标本的腹腔镜左结肠癌根治术(Laparoscopic-assisted Natural Orifice Specimen Extraction,LA-NOSE)围手术期舒适护理经验.舒适护理要点包括:围手术期常规护理:术前心理护理;术前充分的肛门括约肌锻炼和肠道准备;术后肛门功能、胃肠道等的舒适护理;术后加强出院宣教和随访等.结果 LA-NOSE手术患者31例术中未出现大血管、肠管、实质性脏器损伤、中转开腹等并发症.术后均恢复良好,无切口并发症、未对肛门功能造成损伤,康复出院.结论 LA-NOSE手术结合常规护理加舒适护理的护理方法 ,可获得满意的临床护理质量.     

蔡小荪辨治不孕症学术思想及临证经验

总结蔡小荪诊治不孕症的学术思想及临床经验。顺应月经周期调理冲任，辨病与辨证相结合；重视调补肝肾，调畅患者情志；提倡运用经方。     

蔡小荪妇科临证善取通法的经验

中医妇科名医蔡小荪教授临证善取通法。对瘀滞不畅之痛经，通以达之症瘕积聚之子宫肌瘤、子宫内膜异位症，通消兼治；对肾虚，气结，血瘀，痰阻之不孕症，通补相合。     

重楼荠菜生化汤促进人工流产术后子宫复旧的临床研究

目的观察重楼荠菜生化汤对促进人工流产术后子宫复旧的临床效果。方法将I00例进行人工流产术的患者随机分为观察组（重楼荠菜生化汤组）、对照组（益母草胶囊组）。观察组、对照组术后当日分别予以口服重楼荠菜生化汤、益母草胶囊。观察、比较两组病例用药后的子宫复旧情况及安全性。结果观察组在减少人工流产术后阴道流血量、缩短术后阴道流血时间和月经复潮时间、促进子宫缩复及子宫内膜修复等方面均优于对照组,在促进子宫复旧方面疗效显著。观察组患者阴道流血量为（83.12±19.30）g,阴道流血持续时间为（4.74±1.56）d,月经复潮时间为（21.26±4.62）d,子宫三径之和为（17.44±0.97）cm,子宫内膜厚度为（1.20±0.28）cm;对照组患者阴道流血量为（93.60±22.43）g,阴道流血持续时间为（5．92±1．65）d,月经复潮时间为（24.67±5.90）d,子宫三径之和为（18．09±1.22）cm,子宫内膜厚度为（1.03±0．29）cm。观察组在减少人工流产术后阴道流血量、缩短术后阴道流血时间和月经复潮时间、促进子宫恢复及子宫内膜修复等方面均优于对照组。结论重楼荠菜生化汤对于促进人工流产术后子宫复旧方面疗效确切,值得临床上进一步推广应用。     

从《扁鹊心书》浅析窦材的灸法思想

《扁鹊心书》是南宋著名医家窦材所撰.该书是窦氏数十年行医经验的总结,书中着重介绍了有关灸法的临床应用和治验,为灸法的研究发展提供了宝贵的参考材料.笔者就该书中窦材灸法学术思想的特点有：崇素灵、辨经络、治病求本、本于真元;重元阳、温脾肾、扶阳三法、灼艾第一;审虚实、取穴精、辨证证治、艾药齐施;道艾火、法阴阳、灸法养生、延年益寿.     

Effects of Hypoxia on the Growth and Development of the Fetal Ovine Hepatocytes in Primary Culture

Objective To investigate the development and characterizations of the hepatocytes isolated from fetal ovine and to determine the effect of hypoxia on their growth and metabolism.Methods Fresh hepatocytes were isolated from the liver of fetal ovine at late gestation, cultured in specific media, and exposed to normoxia(21% O_2) or hypoxia(2% O_2).The cellular characteristics and population purity were identified by immunocytochemistry and flow cytometry(FCM).The effects of hypoxia on cell cycle and apoptosis of the hepatocytes were evaluated by FCM, whereas the cellular ultrastructure changes were examined with a transmission electron microscope.Results The cell purity of hepatocytes was over 95%.Under hypoxia exposure, the hepatocytes showed a gradual increase in proportion at the S phase and in proliferative index, followed with a compatible increase in apoptosis and progressively decreased cell viability.Additionally, the organelles of the hepatocytes demonstrated dramatic changes, including swelling of mitochondria, disorder in cristae arrangement, expansion of endoplasmic reticulum, and a large number of circular lipid droplets emerging in the cytoplasm.Conclusion Fetal ovine hepatocytes could be primarily cultured in a short-term culture system with a high purity of over 95% and with their preserved original characteristics.Hypoxia could induce changes in ultrastructural and inhibit the proliferation of cultured fetal ovine hepatocytes through apoptotic mechanisms.     

蔡淦治疗慢性胃炎的临床经验

上海市名中医蔡淦教授治疗慢性胃炎经验丰富,认为慢性胃炎的病机关键是肝郁脾虚,治疗强调清胃化湿、养阴增液、活血化瘀、清热解毒、疏肝健脾以及身心调养。